Aging is accompanied by T-cell stiffening and reduced interstitial migration through dysfunctional nuclear organization
No te pierdas la nueva publicación del grupo de Gustavo Plaza en la que se detalla los efectos de los cambios en las propiedades mecánicas de las células T del sistema inmune durante el envejecimiento y su relación con la inmunosenescencia.
Continue ReadingStrategies for the Biofunctionalization of Straining Flow Spinning Regenerated Bombyx mori Fibers
Paloma Lozano-Picazo, Cristina Castro-Domínguez, Augusto Luis Bruno, Alejandro Baeza, Adelia S. Martínez, Patricia A. López, Ángela Castro, Yassmin Lakhal, Elena Montero, Luis Colchero, Daniel González-Nieto, Francisco Javier Rojo, Fivos Panetsos, Milagros Ramos, Rafael Daza, Alfonso M. Gañán-Calvo, Manuel Elices, Gustavo Víctor Guinea and José Pérez-Rigueiro.
Abstract: High-performance regenerated silkworm (Bombyx mori) silk fibers can be produced efficiently through the straining flow spinning (SFS) technique. In addition to an enhanced biocompatibility that results from the removal of contaminants during the processing of the material, regenerated silk fibers may be functionalized conveniently by using a range of different strategies. In this work, the possibility of implementing various functionalization techniques is explored, including the production of fluorescent fibers that may be tracked when implanted, the combination of the fibers with enzymes to yield fibers with catalytic properties, and the functionalization of the fibers with cell-adhesion motifs to modulate the adherence of different cell lineages to the material. When considered globally, all these techniques are a strong indication not only of the high versatility offered by the functionalization of regenerated fibers in terms of the different chemistries that can be employed, but also on the wide range of applications that can be covered with these functionalized fibers.
LINK a la publicación.
Classification of the Binding Events between Streptavidin and Biotin-Decorated Activated Vapour Silanized (Avs) Atomic Force Microscopy Tips
Daniel Corregidor, Raquel Tabraue, Luis Colchero, Rafael Daza, Manuel Elices, Gustavo V. Guinea and José Pérez-Rigueiro.
Abstract: AFM tips were functionalized using the activated vapour silanization (AVS) process and its application in affinity atomic force microscopy was assessed by characterizing the interaction between biotin and streptavidin as model system. AVS-functionalized tips can be easily decorated with sensor molecules following well-established cross-linking chemistries as shown by covalently binding biotin molecules to the amine groups of the AFM tip. Force-distance (F-d) curves were recorded to measure the interaction between the biotin-decorated tips and a substrate covered with covalently bound streptavidin. F-d curves were initially recorded with a pristine streptavidin coating and, subsequently, new F-d curves were obtained after blocking the streptavidin binding sites with biotin-albumin. The F-d curves were classified from the intensity of the adhesion force and the number of interaction events (peaks) observed in each curve. It is shown that single molecular biotin-streptavidin interaction events are recorded consistently and no degradation evidence was observed in any of the functionalized tips during the whole two-step experiment.
LINK a la publicación.
HATs meet structural biology
Échale un vistazo a la nueva publicación del grupo de Oscar Llorca en colaboración con el laboratorio de Manuela Palacin (IRB, Barcelona).
Continue Reading5ª Reunión científica del consorcio Tec4Bio
Con la participación de todos los grupos miembros del Consorcio Tec4Bio-CM, celebramos el pasado jueves 5 de maup nuestra 5ª reunión científica del programa, en esta ocasión acudieron además los miembros del comité evaluador externo. ¡Gracias a todos por vuestra participación!
Continue ReadingLong DNA constructs to study helicases and nucleic acid translocases using optical tweezers
No te pierdas la nueva publicación del grupo de nuestro coordinador Fernando Moreno-Herrero en el que se detalla la fabricación de sustratos largos de DNA para experimentos de biofísica molecular de molécula única.
Continue ReadingAn unexpected role for PD-L1 in front–rear polarization and directional migration
Échale un vistazo al comentario que han publicado desde el grupo MecanoCaveoLab acerca de un nuevo trabajo de investigación sobre PD-L1 (Ligando 1 de muerte programada).
Continue ReadingHuman HELB is a processive motor protein which catalyses RPA clearance from single-stranded DNA
Nueva publicación del grupo de nuestro coordinador Fernando Moreno Herrero (CNB-CSIC) en colaboración con Mark S Dillingham (U. Bristol) donde se caracteriza la actividad de la helicasa humana HELB, implicada en importantes procesos de replicación y reparación del genoma, a través de técnicas bioquímicas y de biofísica de molécula única.
Continue ReadingBasal oxidation of conserved cysteines modulates cardiac titin stiffness and dynamics
No te pierdas el nuevo trabajo del grupo de Jorge Alegre.
Continue ReadingThe mechanics of the heart: zooming in on hypertrophic cardiomyopathy and cMyBP-C
Échale un vistazo a la revisión bibliográfica sobre la miocardiopatía hipertrófica del grupo MOLMECH_CNIC.
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