Molecular architecture and oligomerization of Candida glabrata Cdc13 underpin its telomeric DNA-binding and unfolding activity
Os invitamos a leer la nueva publicación del consorcio Tec4Bio fruto de la colaboración de los grupos de Oscar Llorca y Fernando Moreno-Herrero, junto al laboratorio de Neal F Lue (Weill Cornell Medical College).
Continue ReadingAPLF and long non-coding RNA NIHCOLE promote stable DNA synapsis in non-homologous end joining
No te pierdas la siguiente publicación que ha sido fruto del esfuerzo y la cohesión de 2 grupos del consorcio Tec4Bio junto con investigadores del CIMA (Universidad de Navarra). ¡Enhorabuena a todos los implicados por el trabajo!
Continue ReadingHigh-Yield Characterization of Single Molecule Interactions with DeepTipTM Atomic Force Microscopy Probes
Échale un vistazo a la nueva publicación del grupo de Gustavo Plaza (GMB-UPM).
Continue ReadingCaveolin1-dolines form a distinct and rapid caveolae-independent mechanoadaptation system
Os invitamos a leer la nueva publicación del grupo de Miguel Ángel del Pozo, CNIC.
Continue ReadingA deep learning-based tool for the automated detection and analysis of caveolae in transmission electron microscopy images
Os invitamos a leer la nueva publicación del grupo de Miguel Ángel del Pozo, CNIC.
Continue ReadingCell response to extracellular matrix energy dissipation outweighs rigidity sensing
No te pierdas el siguiente pre-print que ha sido fruto del esfuerzo y la cohesión de 4 grupos del consorcio Tec4Bio. ¡Enhorabuena a todos los implicados por el trabajo!
Continue ReadingStructural basis for the inactivation of cytosolic DNA sensing by the vaccinia virus
Échale un vistazo a la nueva publicación del grupo de Oscar Llorca en colaboración con el laboratorio de Laurence H. Pearl (University of Sussex/Institute of Cancer Research).
Continue ReadingThe Spider Silk Standardization Initiative (S3I): A Powerful Tool to Harness Biological Variability and to Systematize the Characterization of Major Ampullate Silk Fibers Spun by Spiders from Suburban Sydney, Australia
Os invitamos a leer la nueva publicación del grupo de Gustavo Plaza.
Continue ReadingCaveolae couple mechanical stress to integrin recycling and activation
Lolo FN, Pavón DM, Grande A, Elósegui Artola A, Segatori VI, Sánchez S, Trepat X, Roca-Cusachs P, & Del Pozo MA*.
Abstract: Cells are subjected to multiple mechanical inputs throughout their lives. Their ability to detect these environmental cues is called mechanosensing, a process in which integrins play an important role. During cellular mechanosensing, plasma membrane (PM) tension is adjusted to mechanical stress through the buffering action of caveolae; however, little is known about the role of caveolae in early integrin mechanosensing regulation. Here, we show that Cav1KO fibroblasts increase adhesion to FN-coated beads when pulled with magnetic tweezers, as compared to wild type fibroblasts. This phenotype is Rho-independent and mainly derived from increased active β1-integrin content on the surface of Cav1KO fibroblasts. Florescence recovery after photobleaching analysis and endocytosis/recycling assays revealed that active β1-integrin is mostly endocytosed through the clathrin independent carrier/glycosylphosphatidyl inositol (GPI)-enriched endocytic compartment pathway and is more rapidly recycled to the PM in Cav1KO fibroblasts, in a Rab4 and PM tension-dependent manner. Moreover, the threshold for PM tension-driven β1-integrin activation is lower in Cav1KO mouse embryonic fibroblasts (MEFs) than in wild type MEFs, through a mechanism dependent on talin activity. Our findings suggest that caveolae couple mechanical stress to integrin cycling and activation, thereby regulating the early steps of the cellular mechanosensing response.
LINK a la publicación.
Possibilities of using T-cell biophysical biomarkers of aging
Os invitamos a leer la nueva publicación del grupo de Gustavo Plaza sobre los efectos de las propiedades mecánicas de las células T del sistema inmune.
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